plasmids serpine1 Search Results


pai 1 gfp  (Sino Biological)
90
Sino Biological pai 1 gfp
Pai 1 Gfp, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+serpine1/pm36231065-45-2-6?v=Sino+Biological
Average 90 stars, based on 1 article reviews
pai 1 gfp - by Bioz Stars, 2026-08
90/100 stars
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human serpine1  (Addgene inc)
93
Addgene inc human serpine1
The investigated serpins reduce SARS-CoV-2 infection by inhibition of TMPRSS2-mediated spike protein cleavage. (A) HEK-293T cells transfected with the indicated expression plasmids for 24 h were infected with SARS-CoV-2 (MOI = 0.1) for 6 h, and viral RNA was measured by qPCR. (B and C) Posttransfection (24 h) HEK293T cells were infected for 2 h (MOI = 1) and then trypsinized, washed with PBS, and lysed, and the RNA was extracted. Levels of viral RNA were quantified from cDNA synthesized with (B) random hexamers (C) or only the forward primer selectively quantifying the negative sense RNA. Data are cumulative of three independent experiments performed in triplicate; mean and SEM are shown, and statistical significance was calculated by unpaired t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001). (D) Surface plasmon resonance analysis of TMPRSS2 binding to individual serpins. A 2-fold dilution series of TMPRSS2 ranging from 125 nM down to 7.8 nM over immobilized <t>SERPINE1</t> with results shown as response units (RU). Binding kinetics for all serpins are summarized to the right, including the natural target for SERPINE1, tissue plasminogen activator (tPA), as a positive control. (E) TMPRSS2-mediated S-protein cleavage in the presence or absence of individual serpins and the known protease inhibitor nafamostat mesylate. Data from three independent experiments were quantified, and a representative blot is shown. (F) The intensity of bands in panel E corresponding to cleaved S-protein was quantified using ImageJ (Fuji) and normalized to S-protein and TMPRSS2 control. Mean values and SEM are shown; statistical significance was calculated by unpaired t test (*, P < 0.05; **, P < 0.01). (G) HBEC ALI cultures were preincubated apically with recombinant SERPINE1, SERPINA1, or SERPINC1 and infected with SARS-CoV-2 at an MOI of 0.05. The accumulated viral release from the apical side was quantified by qPCR at the indicated time points ( n = 3). Mean and SEM are shown; statistical significance was calculated by unpaired t test (*, P < 0.05; **, P < 0.01). (H) The concentrations of apically released SERPINA1 and SERPINE2 from HBEC ALI cultures from both group high and group low were determined by ELISA. The apical secretions were collected at three time points ( n = 3). Mean and SEM are shown; statistical significance was calculated by unpaired t test (***, P < 0.001).
Human Serpine1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+serpine1/pmc09239148-194-7-10?v=Addgene+inc
Average 93 stars, based on 1 article reviews
human serpine1 - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier
human serpine1 expression plasmid  (Sino Biological)
90
Sino Biological human serpine1 expression plasmid
The investigated serpins reduce SARS-CoV-2 infection by inhibition of TMPRSS2-mediated spike protein cleavage. (A) HEK-293T cells transfected with the indicated expression plasmids for 24 h were infected with SARS-CoV-2 (MOI = 0.1) for 6 h, and viral RNA was measured by qPCR. (B and C) Posttransfection (24 h) HEK293T cells were infected for 2 h (MOI = 1) and then trypsinized, washed with PBS, and lysed, and the RNA was extracted. Levels of viral RNA were quantified from cDNA synthesized with (B) random hexamers (C) or only the forward primer selectively quantifying the negative sense RNA. Data are cumulative of three independent experiments performed in triplicate; mean and SEM are shown, and statistical significance was calculated by unpaired t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001). (D) Surface plasmon resonance analysis of TMPRSS2 binding to individual serpins. A 2-fold dilution series of TMPRSS2 ranging from 125 nM down to 7.8 nM over immobilized <t>SERPINE1</t> with results shown as response units (RU). Binding kinetics for all serpins are summarized to the right, including the natural target for SERPINE1, tissue plasminogen activator (tPA), as a positive control. (E) TMPRSS2-mediated S-protein cleavage in the presence or absence of individual serpins and the known protease inhibitor nafamostat mesylate. Data from three independent experiments were quantified, and a representative blot is shown. (F) The intensity of bands in panel E corresponding to cleaved S-protein was quantified using ImageJ (Fuji) and normalized to S-protein and TMPRSS2 control. Mean values and SEM are shown; statistical significance was calculated by unpaired t test (*, P < 0.05; **, P < 0.01). (G) HBEC ALI cultures were preincubated apically with recombinant SERPINE1, SERPINA1, or SERPINC1 and infected with SARS-CoV-2 at an MOI of 0.05. The accumulated viral release from the apical side was quantified by qPCR at the indicated time points ( n = 3). Mean and SEM are shown; statistical significance was calculated by unpaired t test (*, P < 0.05; **, P < 0.01). (H) The concentrations of apically released SERPINA1 and SERPINE2 from HBEC ALI cultures from both group high and group low were determined by ELISA. The apical secretions were collected at three time points ( n = 3). Mean and SEM are shown; statistical significance was calculated by unpaired t test (***, P < 0.001).
Human Serpine1 Expression Plasmid, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+serpine1/pmc05685717-189-0-12?v=Sino+Biological
Average 90 stars, based on 1 article reviews
human serpine1 expression plasmid - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
serpine1 cdna orf plasmid  (Sino Biological)
91
Sino Biological serpine1 cdna orf plasmid
The sequences of siRNAs and qRT-PCR primers
Serpine1 Cdna Orf Plasmid, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+serpine1/pmc08799265-75-0-7?v=Sino+Biological
Average 91 stars, based on 1 article reviews
serpine1 cdna orf plasmid - by Bioz Stars, 2026-08
91/100 stars
  Buy from Supplier
serpine1 overexpression plasmid  (VectorBuilder GmbH)
90
VectorBuilder GmbH serpine1 overexpression plasmid
The sequences of siRNAs and qRT-PCR primers
Serpine1 Overexpression Plasmid, supplied by VectorBuilder GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmids+serpine1/pm39748408-76-0-12?v=VectorBuilder+GmbH
Average 90 stars, based on 1 article reviews
serpine1 overexpression plasmid - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
pai1 serpine1 human shrna plasmid kit  (OriGene)
N/A
SERPINE1 Human 4 unique 29mer shRNA constructs in retroviral untagged vector
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serpine1 rat shrna plasmid  (OriGene)
N/A
Serpine1 Rat 4 unique 29mer shRNA constructs in lentiviral GFP vector
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serpine1 mouse shrna plasmid  (OriGene)
N/A
Serpine1 Mouse 4 unique 29mer shRNA constructs in lentiviral GFP vector
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rat pai-1 / serpine1 gene orf cdna clone expression plasmid, c-his tag  (Sino Biological)
N/A
Full length Clone DNA of Rat serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 with C terminal His tag.
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rat pai-1 / serpine1 gene orf cdna clone expression plasmid, n-myc tag  (Sino Biological)
N/A
Full length Clone DNA of Rat serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 with N terminal Myc tag.
   Buy from Supplier
rat pai-1 / serpine1 gene orf cdna clone expression plasmid, c-ofpspark tag  (Sino Biological)
N/A
Full length Clone DNA of Rat serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 with C terminal OFPSpark / RFP tag.
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Image Search Results


The investigated serpins reduce SARS-CoV-2 infection by inhibition of TMPRSS2-mediated spike protein cleavage. (A) HEK-293T cells transfected with the indicated expression plasmids for 24 h were infected with SARS-CoV-2 (MOI = 0.1) for 6 h, and viral RNA was measured by qPCR. (B and C) Posttransfection (24 h) HEK293T cells were infected for 2 h (MOI = 1) and then trypsinized, washed with PBS, and lysed, and the RNA was extracted. Levels of viral RNA were quantified from cDNA synthesized with (B) random hexamers (C) or only the forward primer selectively quantifying the negative sense RNA. Data are cumulative of three independent experiments performed in triplicate; mean and SEM are shown, and statistical significance was calculated by unpaired t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001). (D) Surface plasmon resonance analysis of TMPRSS2 binding to individual serpins. A 2-fold dilution series of TMPRSS2 ranging from 125 nM down to 7.8 nM over immobilized SERPINE1 with results shown as response units (RU). Binding kinetics for all serpins are summarized to the right, including the natural target for SERPINE1, tissue plasminogen activator (tPA), as a positive control. (E) TMPRSS2-mediated S-protein cleavage in the presence or absence of individual serpins and the known protease inhibitor nafamostat mesylate. Data from three independent experiments were quantified, and a representative blot is shown. (F) The intensity of bands in panel E corresponding to cleaved S-protein was quantified using ImageJ (Fuji) and normalized to S-protein and TMPRSS2 control. Mean values and SEM are shown; statistical significance was calculated by unpaired t test (*, P < 0.05; **, P < 0.01). (G) HBEC ALI cultures were preincubated apically with recombinant SERPINE1, SERPINA1, or SERPINC1 and infected with SARS-CoV-2 at an MOI of 0.05. The accumulated viral release from the apical side was quantified by qPCR at the indicated time points ( n = 3). Mean and SEM are shown; statistical significance was calculated by unpaired t test (*, P < 0.05; **, P < 0.01). (H) The concentrations of apically released SERPINA1 and SERPINE2 from HBEC ALI cultures from both group high and group low were determined by ELISA. The apical secretions were collected at three time points ( n = 3). Mean and SEM are shown; statistical significance was calculated by unpaired t test (***, P < 0.001).

Journal: mBio

Article Title: Serine Protease Inhibitors Restrict Host Susceptibility to SARS-CoV-2 Infections

doi: 10.1128/mbio.00892-22

Figure Lengend Snippet: The investigated serpins reduce SARS-CoV-2 infection by inhibition of TMPRSS2-mediated spike protein cleavage. (A) HEK-293T cells transfected with the indicated expression plasmids for 24 h were infected with SARS-CoV-2 (MOI = 0.1) for 6 h, and viral RNA was measured by qPCR. (B and C) Posttransfection (24 h) HEK293T cells were infected for 2 h (MOI = 1) and then trypsinized, washed with PBS, and lysed, and the RNA was extracted. Levels of viral RNA were quantified from cDNA synthesized with (B) random hexamers (C) or only the forward primer selectively quantifying the negative sense RNA. Data are cumulative of three independent experiments performed in triplicate; mean and SEM are shown, and statistical significance was calculated by unpaired t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001). (D) Surface plasmon resonance analysis of TMPRSS2 binding to individual serpins. A 2-fold dilution series of TMPRSS2 ranging from 125 nM down to 7.8 nM over immobilized SERPINE1 with results shown as response units (RU). Binding kinetics for all serpins are summarized to the right, including the natural target for SERPINE1, tissue plasminogen activator (tPA), as a positive control. (E) TMPRSS2-mediated S-protein cleavage in the presence or absence of individual serpins and the known protease inhibitor nafamostat mesylate. Data from three independent experiments were quantified, and a representative blot is shown. (F) The intensity of bands in panel E corresponding to cleaved S-protein was quantified using ImageJ (Fuji) and normalized to S-protein and TMPRSS2 control. Mean values and SEM are shown; statistical significance was calculated by unpaired t test (*, P < 0.05; **, P < 0.01). (G) HBEC ALI cultures were preincubated apically with recombinant SERPINE1, SERPINA1, or SERPINC1 and infected with SARS-CoV-2 at an MOI of 0.05. The accumulated viral release from the apical side was quantified by qPCR at the indicated time points ( n = 3). Mean and SEM are shown; statistical significance was calculated by unpaired t test (*, P < 0.05; **, P < 0.01). (H) The concentrations of apically released SERPINA1 and SERPINE2 from HBEC ALI cultures from both group high and group low were determined by ELISA. The apical secretions were collected at three time points ( n = 3). Mean and SEM are shown; statistical significance was calculated by unpaired t test (***, P < 0.001).

Article Snippet: Expression plasmids encoding the precursor ectodomain of human SERPINE1 (SERPINE1-bio-His; Addgene plasmid no. 52077; http://n2t.net/addgene:52077 ; RRID:Addgene_52077), SERPINA1 (SERPINA-bio-His; Addgene plasmid no. 52182; http://n2t.net/addgene:52182 ; RRID:Addgene_52182), and SERPINC1 (SERPINC1-bio-His; Addgene plasmid no. 52076; http://n2t.net/addgene:52076 ; RRID:Addgene_52076) were a kind gift of Gavin Wright ( ).

Techniques: Infection, Inhibition, Transfection, Expressing, Synthesized, SPR Assay, Binding Assay, Positive Control, Protease Inhibitor, Control, Recombinant, Enzyme-linked Immunosorbent Assay

Single-cell RNA sequencing shows distinct expression patterns of serpins in primary lung cells. (A) Schematic illustration of cell types annotated in the single-cell RNA analysis of HBEC ALI cultures. (B) UMAP plot (bidimensional), colored by annotated cell clusters. (C) Coarse-grained graph showing mean cell cluster group expression for SERPINA1, SERPINE1, and SERPINE2. (D) Infection levels analyzed by qPCR and shown as fold increase over 12-h input. (E) Heatmap displaying the mean expression of SERPINA1, SERPINE1, and SERPINE2 upon SARS-CoV-2 infection (±, MOI = 0.05) in HBEC ALI cultures from a group high (H) and a group low (L) donor.

Journal: mBio

Article Title: Serine Protease Inhibitors Restrict Host Susceptibility to SARS-CoV-2 Infections

doi: 10.1128/mbio.00892-22

Figure Lengend Snippet: Single-cell RNA sequencing shows distinct expression patterns of serpins in primary lung cells. (A) Schematic illustration of cell types annotated in the single-cell RNA analysis of HBEC ALI cultures. (B) UMAP plot (bidimensional), colored by annotated cell clusters. (C) Coarse-grained graph showing mean cell cluster group expression for SERPINA1, SERPINE1, and SERPINE2. (D) Infection levels analyzed by qPCR and shown as fold increase over 12-h input. (E) Heatmap displaying the mean expression of SERPINA1, SERPINE1, and SERPINE2 upon SARS-CoV-2 infection (±, MOI = 0.05) in HBEC ALI cultures from a group high (H) and a group low (L) donor.

Article Snippet: Expression plasmids encoding the precursor ectodomain of human SERPINE1 (SERPINE1-bio-His; Addgene plasmid no. 52077; http://n2t.net/addgene:52077 ; RRID:Addgene_52077), SERPINA1 (SERPINA-bio-His; Addgene plasmid no. 52182; http://n2t.net/addgene:52182 ; RRID:Addgene_52182), and SERPINC1 (SERPINC1-bio-His; Addgene plasmid no. 52076; http://n2t.net/addgene:52076 ; RRID:Addgene_52076) were a kind gift of Gavin Wright ( ).

Techniques: RNA Sequencing, Expressing, Infection

The sequences of siRNAs and qRT-PCR primers

Journal: Translational Cancer Research

Article Title: Long noncoding RNA MAFG-AS1 facilitates bladder cancer tumorigenesis via regulation of miR-143-3p/SERPINE1 axis

doi: 10.21037/tcr-20-1971

Figure Lengend Snippet: The sequences of siRNAs and qRT-PCR primers

Article Snippet: SERPINE1 cDNA ORF plasmid was purchased from Sino Biological (Beijing, China).

Techniques: Sequencing

MiR-143-3p targeted MAFG-AS1. (A) Subcellular fractionation analysis displayed that MAFG-AS1was mainly enriched in the cytoplasm. (B) Prediction with starBase 3.0 uncovered the possible binding site for miR-43-3p in MAFG-AS1. (C) Luciferase reporter assay analyzed the luciferase activity of the Wt or Mut MAFG-AS1 reporter in 5637 and T24 cells after transfection of miR-143-3p. (D) RIP assay was performed to examine the interaction between MAFG-AS1, miR-143-3p and SERPINE1. (E) qRT-PCR detected miR-143-3p level in the 52 pairs of bladder cancer samples and matched adjacent normal tissues. (F) Correlation analysis between MAFG-AS1 and miR-143-3p in bladder cancer tissues. (G) MAFG-AS1 silencing could upregulate the expression of miR-143-3p in 5367 and T24 cells. *, P<0.05; **, P<0.01, ***, P<0.001.

Journal: Translational Cancer Research

Article Title: Long noncoding RNA MAFG-AS1 facilitates bladder cancer tumorigenesis via regulation of miR-143-3p/SERPINE1 axis

doi: 10.21037/tcr-20-1971

Figure Lengend Snippet: MiR-143-3p targeted MAFG-AS1. (A) Subcellular fractionation analysis displayed that MAFG-AS1was mainly enriched in the cytoplasm. (B) Prediction with starBase 3.0 uncovered the possible binding site for miR-43-3p in MAFG-AS1. (C) Luciferase reporter assay analyzed the luciferase activity of the Wt or Mut MAFG-AS1 reporter in 5637 and T24 cells after transfection of miR-143-3p. (D) RIP assay was performed to examine the interaction between MAFG-AS1, miR-143-3p and SERPINE1. (E) qRT-PCR detected miR-143-3p level in the 52 pairs of bladder cancer samples and matched adjacent normal tissues. (F) Correlation analysis between MAFG-AS1 and miR-143-3p in bladder cancer tissues. (G) MAFG-AS1 silencing could upregulate the expression of miR-143-3p in 5367 and T24 cells. *, P<0.05; **, P<0.01, ***, P<0.001.

Article Snippet: SERPINE1 cDNA ORF plasmid was purchased from Sino Biological (Beijing, China).

Techniques: Fractionation, Binding Assay, Luciferase, Reporter Assay, Activity Assay, Transfection, Quantitative RT-PCR, Expressing

SERPINE1 was a target of miR-143-3p. (A) Bioinformatics predicted the possible binding site for miR-43-3p in SERPINE1. (B) Luciferase reporter assay analyzed the luciferase activity of the Wt or Mut SERPINE1 reporter in 5637 and T24 cells after transfection of miR-143-3p. (C) The expression of SERPIN1 was markedly reduced in 5367 and T24 cells transfected with miR-143-3p mimics. (D) qRT-PCR detected SERPINE1 level in the 52 pairs of bladder cancer samples and matched adjacent normal tissues. (E) Correlation analysis between SERPINE1 and miR-143-3p in bladder cancer tissues. *, P<0.05.

Journal: Translational Cancer Research

Article Title: Long noncoding RNA MAFG-AS1 facilitates bladder cancer tumorigenesis via regulation of miR-143-3p/SERPINE1 axis

doi: 10.21037/tcr-20-1971

Figure Lengend Snippet: SERPINE1 was a target of miR-143-3p. (A) Bioinformatics predicted the possible binding site for miR-43-3p in SERPINE1. (B) Luciferase reporter assay analyzed the luciferase activity of the Wt or Mut SERPINE1 reporter in 5637 and T24 cells after transfection of miR-143-3p. (C) The expression of SERPIN1 was markedly reduced in 5367 and T24 cells transfected with miR-143-3p mimics. (D) qRT-PCR detected SERPINE1 level in the 52 pairs of bladder cancer samples and matched adjacent normal tissues. (E) Correlation analysis between SERPINE1 and miR-143-3p in bladder cancer tissues. *, P<0.05.

Article Snippet: SERPINE1 cDNA ORF plasmid was purchased from Sino Biological (Beijing, China).

Techniques: Binding Assay, Luciferase, Reporter Assay, Activity Assay, Transfection, Expressing, Quantitative RT-PCR

MAFG-AS1 facilitated bladder cells tumorigenesis by regulating miR-143-3p/SERPINE1 axis. (A) qRT-PCR showed that miR-143-3p inhibition or SERPINE1 overexpression alleviated the MAFG-AS1 silencing-induced suppressed effect on the SERPINE1 expression. MTT (B) and colony formation assay (C) showed that MAFG-AS1 depletion-impeded cells proliferation and growth were obviously attenuated by miR-143-3p inhibitors or SERPINE1 overexpression. Wound scratch (D) and Transwell invasion assay (E) showed that suppressed cell migration and invasion mediated by MAFG-AS1 silencing were significantly alleviated by miR-143-3p inhibition or SERPINE1 overexpression. Scale bars =100 µm. *, P<0.05.

Journal: Translational Cancer Research

Article Title: Long noncoding RNA MAFG-AS1 facilitates bladder cancer tumorigenesis via regulation of miR-143-3p/SERPINE1 axis

doi: 10.21037/tcr-20-1971

Figure Lengend Snippet: MAFG-AS1 facilitated bladder cells tumorigenesis by regulating miR-143-3p/SERPINE1 axis. (A) qRT-PCR showed that miR-143-3p inhibition or SERPINE1 overexpression alleviated the MAFG-AS1 silencing-induced suppressed effect on the SERPINE1 expression. MTT (B) and colony formation assay (C) showed that MAFG-AS1 depletion-impeded cells proliferation and growth were obviously attenuated by miR-143-3p inhibitors or SERPINE1 overexpression. Wound scratch (D) and Transwell invasion assay (E) showed that suppressed cell migration and invasion mediated by MAFG-AS1 silencing were significantly alleviated by miR-143-3p inhibition or SERPINE1 overexpression. Scale bars =100 µm. *, P<0.05.

Article Snippet: SERPINE1 cDNA ORF plasmid was purchased from Sino Biological (Beijing, China).

Techniques: Quantitative RT-PCR, Inhibition, Over Expression, Expressing, Colony Assay, Transwell Invasion Assay, Migration